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Creutzfeldt-Jakob Disease (CJD)

VERSION: 1.1 CREATED: January 30, 2026 REVISED: January 30, 2026 STATUS: Approved


DIAGNOSIS: Creutzfeldt-Jakob Disease (CJD)

ICD-10: A81.00 (Creutzfeldt-Jakob disease, unspecified), A81.01 (Variant Creutzfeldt-Jakob disease), A81.09 (Other Creutzfeldt-Jakob disease), A81.1 (Subacute sclerosing panencephalitis), A81.9 (Atypical virus infections of CNS, unspecified)

CPT CODES: 70553 (MRI brain with and without contrast), 95816 (EEG routine), 95950 (cEEG monitoring), 89051 (CSF cell count), 84157 (CSF protein), 82945 (CSF glucose), 83519 (14-3-3 protein, tau, NfL), 86235 (RT-QuIC), 81479 (PRNP gene sequencing), 88305 (Brain biopsy pathology), 70450 (CT head without contrast)

SYNONYMS: Creutzfeldt-Jakob disease, CJD, sporadic CJD, sCJD, variant CJD, vCJD, familial CJD, fCJD, iatrogenic CJD, iCJD, prion disease, transmissible spongiform encephalopathy, TSE, mad cow disease, bovine spongiform encephalopathy related, BSE-related CJD, spongiform encephalopathy, prion encephalopathy, Jakob-Creutzfeldt disease, subacute spongiform encephalopathy, rapid cognitive decline with myoclonus, prion dementia, human prion disease

SCOPE: Diagnostic workup, symptomatic management, and palliative care for suspected or confirmed Creutzfeldt-Jakob disease across all subtypes (sporadic, familial/genetic, iatrogenic, variant). Covers WHO/CDC diagnostic criteria application, biomarker interpretation (RT-QuIC, 14-3-3, tau), neuroimaging (MRI DWI cortical ribboning), EEG (periodic sharp wave complexes), PRNP genotyping, myoclonus management, infection control (prion decontamination), palliative care initiation, family counseling, genetic counseling for familial forms, and autopsy/National Prion Disease Pathology Surveillance Center referral. No disease-modifying treatment exists -- management is entirely supportive and palliative. For comprehensive RPD evaluation including treatable mimics, use the "Rapidly Progressive Dementia" template. For autoimmune encephalitis (key differential), use the "Autoimmune Encephalitis" template.


DEFINITIONS:

  • Sporadic CJD (sCJD): Most common form (~85-90% of cases); arises spontaneously without identifiable risk factor; median age of onset 62 years; median survival 5-6 months
  • Familial/Genetic CJD (fCJD): ~10-15% of cases; autosomal dominant PRNP mutations (E200K most common); includes familial CJD, Gerstmann-Straussler-Scheinker syndrome (GSS), and fatal familial insomnia (FFI)
  • Iatrogenic CJD (iCJD): Transmission via contaminated surgical instruments, corneal transplants, dura mater grafts, human-derived growth hormone; extremely rare since modern precautions
  • Variant CJD (vCJD): Linked to bovine spongiform encephalopathy (BSE/"mad cow disease"); younger onset (median age 28); prominent psychiatric symptoms early; "pulvinar sign" on MRI; primarily in UK/Europe; extremely rare currently

PRIORITY KEY: STAT = Immediate | URGENT = Within hours | ROUTINE = Standard | EXT = Extended/atypical cases | - = Not applicable to this setting

═══════════════════════════════════════════════════════════════ SECTION A: ACTION ITEMS ═══════════════════════════════════════════════════════════════

1. LABORATORY WORKUP

1A. Essential/Core Labs

Test Rationale Target Finding ED HOSP OPD ICU
CBC with differential (CPT 85025) Baseline; infection screen; rule out hematologic causes of encephalopathy Normal STAT STAT ROUTINE STAT
CMP (CPT 80053) Metabolic encephalopathy screen (hepatic, renal, electrolyte); baseline before medications Normal STAT STAT ROUTINE STAT
TSH (CPT 84443), free T4 (CPT 84439) Hashimoto encephalopathy (SREAT) is a treatable mimic; hypothyroid encephalopathy Normal URGENT ROUTINE ROUTINE URGENT
B12 (CPT 82607), methylmalonic acid (MMA) (CPT 83921) B12 deficiency is a reversible cause of cognitive decline; rule out before accepting prion diagnosis B12 >300 pg/mL; MMA normal URGENT ROUTINE ROUTINE URGENT
ESR (CPT 85652) Vasculitis screen; inflammatory conditions; normal in CJD (helps exclude autoimmune/inflammatory mimics) Normal (<20 mm/h age-adjusted) URGENT ROUTINE ROUTINE URGENT
CRP (CPT 86140) Inflammatory marker; elevated suggests non-prion etiology (autoimmune, infection, vasculitis) Normal URGENT ROUTINE ROUTINE URGENT
RPR or VDRL (CPT 86592) Neurosyphilis is a treatable RPD mimic Non-reactive URGENT ROUTINE ROUTINE URGENT
HIV 1/2 antigen/antibody (CPT 87389) HIV-associated neurocognitive disorder; opportunistic infections Negative URGENT ROUTINE ROUTINE URGENT
Ammonia (CPT 82140) Hepatic encephalopathy mimic Normal (<35 micromol/L) URGENT ROUTINE - URGENT
Blood glucose (CPT 82947) Hypoglycemia; diabetic encephalopathy Normal STAT STAT ROUTINE STAT
Urinalysis (CPT 81003) + urine culture UTI causing delirium superimposed on CJD Negative STAT ROUTINE ROUTINE STAT
Urine drug screen Substance-related cognitive impairment; drug toxicity Negative or expected medications only URGENT ROUTINE - URGENT

1B. Extended Workup (Second-line)

Test Rationale Target Finding ED HOSP OPD ICU
Anti-thyroid antibodies (anti-TPO, anti-thyroglobulin) Hashimoto encephalopathy (SREAT) -- treatable with steroids; can mimic CJD Negative (positive + encephalopathy = consider SREAT) - ROUTINE ROUTINE -
ANA (CPT 86235) Lupus cerebritis; systemic autoimmune disease mimicking RPD Negative - ROUTINE ROUTINE -
Autoimmune encephalitis panel (serum) -- NMDAR, LGI1, CASPR2, GABA-B, GABA-A, AMPA, DPPX, IgLON5 Treatable autoimmune encephalitis is the most important reversible CJD mimic to identify Negative - ROUTINE ROUTINE -
Paraneoplastic panel (serum) -- comprehensive (ANNA-1/Hu, ANNA-2/Ri, CRMP-5, amphiphysin, PCA-1/Yo, GAD65) Paraneoplastic encephalitis can mimic CJD presentation Negative - ROUTINE ROUTINE -
Folate (CPT 82746) Deficiency associated with cognitive impairment Normal - ROUTINE ROUTINE -
LDH Lymphoma; intravascular lymphoma (CJD mimic) Normal - ROUTINE ROUTINE -
Peripheral blood smear Intravascular lymphoma (schistocytes); TTP Normal - ROUTINE - -
ACE level (serum) Neurosarcoidosis Normal - ROUTINE ROUTINE -
Serum protein electrophoresis (SPEP) (CPT 86334) + immunofixation CNS lymphoma; POEMS; paraneoplastic Normal pattern - ROUTINE ROUTINE -
Thiamine (B1) level (CPT 84425) Wernicke encephalopathy -- reversible with treatment Normal (>70 nmol/L) - ROUTINE ROUTINE -
Copper (CPT 82390) and ceruloplasmin Wilson disease (if age <50) Normal - ROUTINE ROUTINE -

1C. Rare/Specialized (Prion-Specific and Genetic)

Test Rationale Target Finding ED HOSP OPD ICU
PRNP gene analysis (blood) (CPT 81479) Identifies genetic/familial prion disease (fCJD, GSS, FFI); determines codon 129 polymorphism (methionine/valine) which influences sCJD phenotype and prognosis; important for family counseling No pathogenic mutation; document codon 129 genotype (MM, MV, VV) - ROUTINE ROUTINE -
Codon 129 polymorphism (PRNP) MM homozygosity most common in sCJD-MM1 (classic rapid form); MV and VV associated with different clinical phenotypes and prognosis MM most common in sCJD; VV/MV in some atypical forms - ROUTINE ROUTINE -
Neurofilament light chain (NfL) (CPT 83519) -- serum Markedly elevated in CJD; useful as accessible blood biomarker of neuronal damage; may support diagnosis when CSF unavailable Markedly elevated (>10,000 pg/mL in CJD) - ROUTINE ROUTINE -
Prion protein gene full sequencing If PRNP targeted analysis negative but clinical suspicion for genetic prion disease remains high No pathogenic variant - EXT EXT -
AD biomarkers (serum p-tau217, p-tau181) Rapidly progressive Alzheimer disease is the most common misdiagnosis of CJD; serum AD biomarkers help distinguish Normal p-tau ratio (elevated suggests rpAD, not CJD) - ROUTINE ROUTINE -

2. DIAGNOSTIC IMAGING & STUDIES

2A. Essential/First-line

Study Timing Target Finding Contraindications ED HOSP OPD ICU
MRI brain with and without contrast (CPT 70553) + DWI/FLAIR Within 24h. DWI is CRITICAL -- cortical ribboning on DWI is highly characteristic of CJD. 3T preferred for sensitivity sCJD: cortical ribboning on DWI (high signal in neocortex) + caudate/putamen signal on DWI/FLAIR. vCJD: pulvinar sign (bilateral pulvinar thalamic high signal). fCJD: variable by mutation. Sensitivity of MRI DWI for sCJD >90% Pacemaker; metallic implants; severe claustrophobia STAT STAT ROUTINE STAT
EEG (routine) (CPT 95816) Within 24h sCJD: periodic sharp wave complexes (PSWCs) at 1-2 Hz -- triphasic morphology; generalized; sensitivity ~65% (highest in sCJD-MM1 subtype). Also rules out non-convulsive status epilepticus None significant URGENT URGENT ROUTINE URGENT
CT head without contrast (CPT 70450) Immediate if acute presentation, focal deficit, or pre-LP Mass lesion; hydrocephalus; hemorrhage; CT is typically normal early in CJD but may show atrophy in later stages Pregnancy (relative) STAT STAT - STAT

2B. Extended

Study Timing Target Finding Contraindications ED HOSP OPD ICU
Continuous EEG monitoring (cEEG) (CPT 95700) If altered consciousness, suspected NCSE, or fluctuating exam Non-convulsive seizures; evolution of PSWCs; helps distinguish CJD periodic pattern from seizures None - URGENT - STAT
FDG-PET brain (CPT 78608) Within 1-2 weeks CJD: cortical and striatal hypometabolism (often asymmetric early); helps distinguish from AD pattern (temporoparietal); may be useful when MRI is equivocal Pregnancy; uncontrolled diabetes - EXT ROUTINE -
MRA brain If vasculitis suspected as alternative diagnosis CNS vasculitis (beading); normal in CJD Same as MRI - ROUTINE ROUTINE -
CT chest/abdomen/pelvis with contrast If paraneoplastic syndrome suspected Occult malignancy (paraneoplastic RPD mimic) Contrast allergy; renal impairment - ROUTINE ROUTINE -
Sleep polysomnography If fatal familial insomnia (FFI) suspected Absent sleep spindles; disrupted sleep architecture; progressive total insomnia Cooperation required - - EXT -

2C. Rare/Specialized

Study Timing Target Finding Contraindications ED HOSP OPD ICU
Brain biopsy When all non-invasive testing inconclusive and treatable etiology must be excluded; NOT required for CJD diagnosis if RT-QuIC positive and MRI/EEG consistent Spongiform change; PrP immunostaining (definite CJD); also rules out vasculitis, lymphoma, autoimmune Coagulopathy; surgical risk; biopsy only changes management if treatable diagnosis possible - EXT - -
Second opinion MRI neuroradiology review If initial MRI read as normal but clinical suspicion for CJD remains high Subtle DWI cortical signal missed on initial read; CJD MRI findings require expertise None - ROUTINE ROUTINE -
Nasal brushing for RT-QuIC Emerging alternative to CSF RT-QuIC; non-invasive; sensitivity 97% in some studies Positive RT-QuIC from olfactory mucosa supports prion diagnosis None significant; early-stage research - EXT EXT -

LUMBAR PUNCTURE

Indication: Essential for CJD diagnostic workup. CSF RT-QuIC is the gold standard biomarker for prion disease (sensitivity 92%, specificity 99-100%). Also needed to exclude treatable RPD mimics (autoimmune encephalitis, infection, malignancy).

Timing: URGENT. Perform early in workup after CT head rules out contraindications. Do not delay for MRI.

Volume Required: 15-20 mL (multiple specialized tests required; save extra frozen at -80 degrees C for future studies)

Study Rationale Target Finding ED HOSP OPD ICU
Opening pressure Elevated ICP assessment; typically normal in CJD Normal (10-20 cm H2O); normal in CJD URGENT ROUTINE ROUTINE URGENT
Cell count with differential (tubes 1 and 4) (CPT 89051) Normal or minimal pleocytosis in CJD; elevated WBC suggests autoimmune or infectious etiology Normal in CJD (WBC <5); elevated WBC suggests non-prion diagnosis URGENT ROUTINE ROUTINE URGENT
Protein (CPT 84157) Mildly elevated or normal in CJD; markedly elevated suggests infection/autoimmune Normal or mildly elevated in CJD (usually <100 mg/dL) URGENT ROUTINE ROUTINE URGENT
Glucose with paired serum glucose (CPT 82945) Low in bacterial/TB/fungal meningitis, carcinomatous meningitis; normal in CJD Normal in CJD (>60% of serum) URGENT ROUTINE ROUTINE URGENT
Gram stain and bacterial culture (CPT 87205+87070) Exclude chronic bacterial infection No organisms URGENT ROUTINE ROUTINE URGENT
RT-QuIC (real-time quaking-induced conversion) (CPT 86235) GOLD STANDARD for CJD diagnosis. Sensitivity 92%, specificity 99-100%. Detects misfolded prion protein amplification. Send to National Prion Disease Pathology Surveillance Center (Cleveland) or Quest/Mayo Negative (positive = prion disease essentially confirmed) URGENT URGENT ROUTINE URGENT
14-3-3 protein (CPT 83519) Elevated in rapid neuronal destruction. Sensitivity ~90% for sCJD but NOT specific (also elevated in stroke, encephalitis, seizures, CNS lymphoma). Largely superseded by RT-QuIC Negative (positive supports CJD in right clinical context but has significant false-positive rate) URGENT ROUTINE ROUTINE URGENT
Total tau protein (CPT 83519) Markedly elevated in CJD (>1150 pg/mL has sensitivity 90%, specificity 86%). Higher levels correlate with more rapid progression. Not specific (also elevated in AD, stroke) Normal (<400 pg/mL); CJD often >1150, frequently >10,000 pg/mL URGENT ROUTINE ROUTINE URGENT
Neuron-specific enolase (NSE) Elevated in CJD (>35 ng/mL); marker of neuronal damage; less specific than tau or RT-QuIC Normal (<35 ng/mL); elevated in CJD - ROUTINE ROUTINE -
Neurofilament light chain (NfL) (CPT 83519) -- CSF Markedly elevated in CJD; non-specific marker of neuronal damage; useful for tracking progression Markedly elevated in CJD (often >10,000 pg/mL) - ROUTINE ROUTINE -
Autoimmune encephalitis panel (CSF) -- NMDAR, LGI1, CASPR2, GABA-B, GABA-A, AMPA, DPPX CSF more sensitive than serum for NMDAR antibodies; rule out treatable autoimmune encephalitis before accepting CJD diagnosis Negative URGENT ROUTINE ROUTINE URGENT
Alzheimer biomarkers (CSF Abeta42, Abeta40, p-tau 181, t-tau) (CPT 83519) Rapidly progressive AD (rpAD) is the most common misdiagnosis of CJD. AD pattern: low Abeta42/Abeta40 ratio + elevated p-tau. In CJD: t-tau markedly elevated but p-tau NOT proportionally elevated (p-tau/t-tau ratio very low) Context-dependent; p-tau/t-tau ratio helps distinguish CJD from rpAD - ROUTINE ROUTINE -
Oligoclonal bands (CPT 83916), IgG index Intrathecal antibody synthesis (MS, autoimmune, neurosarcoidosis); typically absent in CJD Absent in CJD; present suggests inflammatory/autoimmune etiology - ROUTINE ROUTINE -
HSV 1/2 PCR (CPT 87529) HSV encephalitis mimic Negative URGENT ROUTINE - URGENT
Cytology (CPT 88104) Leptomeningeal carcinomatosis/lymphoma (RPD mimic) Negative - ROUTINE ROUTINE -
Flow cytometry CNS lymphoma (B-cell clonality) Normal - ROUTINE ROUTINE -
VDRL (CSF) (CPT 86592) Neurosyphilis Non-reactive - ROUTINE ROUTINE -
Cryptococcal antigen (CPT 87327) Cryptococcal meningitis (immunocompromised) Negative URGENT ROUTINE - URGENT

Special Handling: RT-QuIC requires specific collection and shipping procedures -- contact National Prion Disease Pathology Surveillance Center (1-216-368-0587) or reference lab for specimen requirements. Send CSF on dry ice. Standard prion precautions NOT required for CSF handling (prion concentration in CSF is very low). Save 5-10 mL of CSF frozen at -80 degrees C for future studies.

Contraindications: Elevated ICP without imaging (get CT first); coagulopathy (INR >1.5, platelets <50K); skin infection at LP site; posterior fossa mass with herniation risk


3. TREATMENT

There is NO disease-modifying treatment for CJD. All management is symptomatic and palliative. The primary goals are comfort, dignity, seizure/myoclonus control, and family support.

3A. Acute/Emergent

Treatment Route Indication Dosing Contraindications Monitoring ED HOSP OPD ICU
Lorazepam (acute seizure) IV Acute seizure or status epilepticus 4 mg :: IV :: PRN seizure :: 0.1 mg/kg IV (max 4 mg/dose); may repeat x1 in 5 min; max 8 mg total Respiratory depression; acute narrow-angle glaucoma Respiratory status; sedation level; airway patency STAT STAT - STAT
Levetiracetam (seizure management) IV Seizure prophylaxis and treatment in CJD; preferred first-line ASM due to minimal drug interactions 1000 mg :: IV :: BID :: Load 1000-1500 mg IV; maintenance 500-1500 mg PO/IV BID; max 3000 mg/day Severe renal impairment (dose adjust per CrCl) Behavioral changes; renal function; seizure frequency STAT STAT ROUTINE STAT
Thiamine (B1) IV -- empiric IV Empiric for Wernicke encephalopathy while RPD workup pending; treat before glucose 500 mg :: IV :: TID :: 500 mg IV TID x 3 days, then 250 mg IV daily x 3-5 days, then 100 mg PO daily None significant Clinical response (confusion, ataxia, ophthalmoplegia) STAT STAT - STAT
IV methylprednisolone (empiric trial) IV Empiric immunotherapy trial ONLY if autoimmune encephalitis remains in differential; do NOT continue if CJD confirmed (steroids do not help CJD) 1000 mg :: IV :: daily :: 1000 mg IV daily x 3-5 days; infuse over 1-2 hours; DISCONTINUE if CJD confirmed Active untreated infection; CJD confirmed (no benefit); uncontrolled diabetes Glucose q6h; BP; GI prophylaxis; clinical response assessment - URGENT - URGENT

Note: Empiric immunotherapy should be considered ONLY when autoimmune encephalitis has not been ruled out. If RT-QuIC is positive or clinical/imaging profile strongly favors CJD, steroids provide no benefit and should not be given. The critical decision point is whether treatable causes have been adequately excluded.

3B. Symptomatic Treatments -- Myoclonus

Treatment Route Indication Dosing Contraindications Monitoring ED HOSP OPD ICU
Clonazepam (first-line for myoclonus) PO Myoclonus -- most effective treatment for CJD-associated myoclonus; reduces startle response and involuntary jerking 0.5 mg :: PO :: BID :: Start 0.5 mg PO BID; increase by 0.5 mg every 3-5 days as tolerated; target 1-2 mg TID; max 6 mg/day Respiratory depression; severe hepatic impairment; fall risk (elderly); acute narrow-angle glaucoma Sedation level; respiratory status; fall risk assessment; swallowing function (may worsen dysphagia) URGENT STAT ROUTINE STAT
Valproic acid (adjunctive for myoclonus) PO Myoclonus and seizure control -- add to or substitute for clonazepam if insufficient myoclonus control or excessive sedation from benzodiazepines 250 mg :: PO :: BID :: Start 250 mg PO BID; increase by 250 mg every 3-5 days; target level 50-100 mcg/mL; max 2000 mg/day Hepatic disease; pancreatitis; urea cycle disorders; pregnancy (teratogenic) LFTs; ammonia; CBC (thrombocytopenia); drug level q1-2 weeks until stable; pancreatitis symptoms URGENT ROUTINE ROUTINE URGENT
Levetiracetam (adjunctive for myoclonus) PO Myoclonus -- alternative or adjunct when benzodiazepine sedation is limiting; also provides seizure coverage 500 mg :: PO :: BID :: Start 500 mg PO BID; increase by 500 mg/day every 1-2 weeks; max 3000 mg/day Severe renal impairment (dose adjust per CrCl) Behavioral changes (irritability, aggression); renal function; seizure/myoclonus diary - ROUTINE ROUTINE ROUTINE
Piracetam (adjunctive for myoclonus) PO Cortical myoclonus -- may be effective for CJD myoclonus; not FDA-approved in US but available internationally 2400 mg :: PO :: TID :: Start 2400 mg PO TID; may increase to 4800 mg TID; max 24 g/day Severe renal impairment; cerebral hemorrhage Renal function; clinical response - EXT EXT -

3C. Symptomatic Treatments -- Behavioral and Other Symptoms

Treatment Route Indication Dosing Contraindications Monitoring ED HOSP OPD ICU
Quetiapine (agitation/psychosis) PO Agitation, psychosis, behavioral disturbance in CJD -- preferred atypical antipsychotic due to lower EPS risk 25 mg :: PO :: qHS :: Start 25 mg PO qHS; increase by 25 mg/day every 2-3 days; max 200-400 mg/day QTc prolongation; Lewy body dementia (must distinguish from CJD -- DLB has worse antipsychotic sensitivity) QTc; metabolic panel; sedation; fall risk; EPS monitoring - ROUTINE ROUTINE ROUTINE
Haloperidol (acute severe agitation) IV Acute severe agitation or psychosis not responsive to reorientation and non-pharmacologic measures 0.5 mg :: IV :: PRN agitation :: 0.5-2 mg IV/IM q4-6h PRN agitation; use lowest effective dose; max 10 mg/day QTc >500 ms; Parkinson disease; neuroleptic malignant syndrome history ECG (QTc before and after); EPS; NMS surveillance (temperature, rigidity, CPK) STAT URGENT - STAT
Trazodone (insomnia) PO Sleep disruption and insomnia -- common in CJD; preferred over benzodiazepine hypnotics to avoid excessive sedation 25 mg :: PO :: qHS :: Start 25 mg PO qHS; increase by 25 mg every 3-5 days; max 150 mg qHS Concurrent MAOIs; QTc prolongation; priapism risk Sedation; orthostatic hypotension; swallowing safety - ROUTINE ROUTINE -
Melatonin (insomnia) PO Sleep-wake cycle disruption -- may be tried first given excellent safety profile 3 mg :: PO :: qHS :: 3-10 mg PO qHS; start at 3 mg None significant Sleep quality - ROUTINE ROUTINE -
Morphine (pain and distress) IV Pain, suffering, and distress in advanced CJD -- palliative intent; myoclonus can be painful 2 mg :: IV :: q4h PRN :: Start 1-2 mg IV q2-4h PRN; titrate to comfort; convert to scheduled dosing if frequent PRN use Respiratory depression (acceptable in comfort-focused care); severe hepatic impairment Respiratory rate; comfort level; sedation; bowel function (constipation prophylaxis) - ROUTINE - ROUTINE
Morphine oral/sublingual (outpatient palliative) PO Pain and distress management in home hospice setting 5 mg :: PO :: q4h PRN :: Immediate release: 5-10 mg PO q4h PRN; convert to sustained-release when dose established; sublingual concentrated solution for dysphagia Respiratory depression (acceptable in comfort-focused care); severe hepatic impairment Respiratory rate; comfort level; sedation; bowel function; stool softener/laxative prophylaxis - ROUTINE ROUTINE -
Glycopyrrolate (sialorrhea) PO Excessive drooling/sialorrhea -- common in advanced CJD with bulbar dysfunction 1 mg :: PO :: TID :: Start 1 mg PO TID; increase to 2 mg TID as needed Narrow-angle glaucoma; urinary retention; GI obstruction Anticholinergic effects; urinary retention; constipation; dry mouth - ROUTINE ROUTINE -
Hyoscine (scopolamine) patch (sialorrhea) TOP Alternative for sialorrhea management when oral medications difficult due to dysphagia 1.5 mg :: TOP :: q72h :: Apply 1 transdermal patch behind ear every 72 hours; rotate ears Narrow-angle glaucoma; urinary retention Confusion (may worsen); dry mouth; urinary retention - ROUTINE ROUTINE -
Ondansetron (nausea) IV Nausea and vomiting -- may occur with brainstem involvement or medication side effects 4 mg :: IV :: q8h PRN :: 4 mg IV q8h PRN; or 4-8 mg PO q8h PRN QTc prolongation; severe hepatic impairment QTc if repeated dosing URGENT ROUTINE ROUTINE URGENT
Docusate + senna (constipation prophylaxis) PO Constipation prevention -- universal need with immobility and opioid use 100 mg :: PO :: BID :: Docusate 100 mg PO BID + senna 8.6 mg PO BID; titrate senna to effect Bowel obstruction Bowel frequency; abdominal distension - ROUTINE ROUTINE -
Polyethylene glycol (PEG 3350) (constipation) PO Constipation refractory to docusate/senna 17 g :: PO :: daily :: 17 g PO daily in 8 oz water; may increase to BID Bowel obstruction; ileus Bowel frequency; electrolytes if prolonged use - ROUTINE ROUTINE -

3D. Palliative Care Interventions

Treatment Route Indication Dosing Contraindications Monitoring ED HOSP OPD ICU
Comfort-focused care transition - All confirmed CJD patients -- initiate goals of care discussion at diagnosis; median survival 5-6 months for sCJD - :: - :: - :: Goals of care discussion; advance directive completion; POLST/MOLST completion; hospice referral when appropriate None Goals of care revisited at each visit; functional status; caregiver burden URGENT STAT ROUTINE STAT
Hospice enrollment - Prognosis <6 months (applies to most sCJD at diagnosis); patient/family ready for comfort-focused approach - :: - :: - :: Per hospice agency; home hospice preferred if safe; inpatient hospice if symptoms uncontrolled or caregiver unable Patient/family not ready; still pursuing curative workup for non-CJD diagnosis Comfort; family coping; symptom control - ROUTINE ROUTINE -
Discontinuation of non-essential medications PO Medication reconciliation -- stop statins, antihypertensives, diabetes medications, preventive therapies that no longer serve goals of care - :: - :: - :: Gradual taper or discontinuation per clinical judgment Do not abruptly stop benzodiazepines, opioids, or anticonvulsants (taper if discontinuing) Comfort; symptom control after medication changes - ROUTINE ROUTINE -

4. OTHER RECOMMENDATIONS

4A. Referrals & Consults

Recommendation ED HOSP OPD ICU
Neurology (prion/neurodegenerative specialist if available) for diagnostic confirmation, prognostication, and ongoing management STAT STAT STAT STAT
Palliative care for goals of care discussion, symptom management, advance care planning, and hospice referral coordination URGENT STAT ROUTINE STAT
National Prion Disease Pathology Surveillance Center (NPDPSC, Case Western Reserve University, Cleveland, OH; phone 1-216-368-0587) for diagnostic guidance, RT-QuIC testing, and potential autopsy coordination - ROUTINE ROUTINE -
Genetic counseling for familial CJD evaluation, PRNP testing implications, and family member risk assessment - ROUTINE ROUTINE -
Psychiatry for behavioral symptom management, capacity evaluation, and caregiver psychiatric support - ROUTINE ROUTINE -
Social work for advance directive facilitation, caregiver support, insurance/disability assistance, and community resources - ROUTINE ROUTINE -
Ethics consultation for end-of-life decision-making, especially if family conflict about goals of care or capacity questions - ROUTINE - ROUTINE
Speech-language pathology for dysphagia evaluation and aspiration risk assessment to guide feeding recommendations - ROUTINE ROUTINE -
Physical therapy for safety assessment, mobility aids, and fall prevention as motor function declines - ROUTINE ROUTINE -
Occupational therapy for ADL adaptation, caregiver training for safe transfers, and home safety evaluation - ROUTINE ROUTINE -
Chaplain/spiritual care for patient and family spiritual support during terminal diagnosis - ROUTINE ROUTINE -
Infection control/hospital epidemiology for prion decontamination protocols and staff education - ROUTINE - ROUTINE
CJD Foundation (www.cjdfoundation.org) referral for patient and family support, educational resources, and peer connections - ROUTINE ROUTINE -

4B. Patient and Family Instructions

Recommendation ED HOSP OPD ICU
CJD is NOT contagious through casual contact, sharing meals, kissing, or caring for the patient -- standard household precautions are sufficient; no isolation needed at home ROUTINE ROUTINE ROUTINE ROUTINE
CJD has no cure or disease-modifying treatment; management focuses entirely on comfort, symptom control, and quality of life ROUTINE ROUTINE ROUTINE ROUTINE
Return to ED immediately if new seizures, choking/aspiration, inability to swallow, high fever, or sudden worsening of symptoms STAT STAT ROUTINE -
Complete advance directives and healthcare power of attorney EARLY while patient can still participate in decision-making (capacity declines rapidly in CJD) URGENT URGENT ROUTINE URGENT
Do NOT drive at any point after CJD diagnosis due to progressive cognitive and motor impairment ROUTINE ROUTINE ROUTINE -
Ensure 24/7 supervision due to fall risk, wandering, impaired judgment, and progressive functional decline - ROUTINE ROUTINE ROUTINE
Notify all surgical, dental, and medical providers of CJD diagnosis -- special instrument sterilization or single-use instruments required for any invasive procedure - ROUTINE ROUTINE ROUTINE
Blood, organ, and tissue donation is NOT permitted with CJD diagnosis - ROUTINE ROUTINE ROUTINE
Contact the CJD Foundation (1-800-659-1991 or www.cjdfoundation.org) for family support resources, educational materials, and peer support groups - ROUTINE ROUTINE -
Autopsy is strongly encouraged for definitive diagnosis, disease surveillance, and scientific research -- discuss with NPDPSC early; autopsy is provided at no cost through the Surveillance Center - ROUTINE ROUTINE -
Familial CJD: first-degree relatives should be offered genetic counseling and PRNP testing if mutation identified in proband - ROUTINE ROUTINE -

4C. Infection Control, Safety & Lifestyle

Recommendation ED HOSP OPD ICU
Standard precautions only for routine patient care (gloves, gown as needed); CJD is NOT transmitted by respiratory droplets, blood, urine, or feces in normal care ROUTINE ROUTINE ROUTINE ROUTINE
Prion decontamination for surgical instruments: instruments contacting high-infectivity tissues (brain, spinal cord, eye) must be destroyed or undergo WHO-recommended decontamination (1N NaOH for 1 hour followed by autoclaving at 134 degrees C for 1 hour in a gravity displacement autoclave) ROUTINE ROUTINE ROUTINE ROUTINE
Standard sterilization is INSUFFICIENT for prion decontamination -- conventional autoclaving, ethylene oxide, formaldehyde, alcohol, and UV do not reliably inactivate prions ROUTINE ROUTINE ROUTINE ROUTINE
Single-use disposable instruments recommended for any invasive procedure on confirmed or suspected CJD patients ROUTINE ROUTINE ROUTINE ROUTINE
Avoid LP needle reuse; dispose of all LP supplies as biohazard (standard practice) ROUTINE ROUTINE - ROUTINE
CSF handling: standard laboratory precautions are adequate; prion concentration in CSF is very low; gloves and eye protection for splash risk ROUTINE ROUTINE ROUTINE ROUTINE
Endoscopy in CJD patients: use disposable accessories where possible; standard endoscope reprocessing may be insufficient for prion decontamination -- consult infection control - ROUTINE ROUTINE ROUTINE
Fall prevention measures: bed alarm, non-slip surfaces, grab bars, low bed, hip protectors for patients with gait instability and myoclonus ROUTINE ROUTINE ROUTINE ROUTINE
Aspiration precautions: head of bed elevated 30 degrees; thickened liquids if dysphagia present; SLP evaluation for safe diet recommendations - ROUTINE ROUTINE ROUTINE
Skin integrity: frequent repositioning every 2 hours; pressure-relieving mattress; skin assessment daily for immobile patients - ROUTINE ROUTINE ROUTINE
Caregiver self-care: encourage caregivers to maintain their own medical appointments, sleep hygiene, nutrition, and social connections; connect with respite care services and CJD Foundation peer support groups - ROUTINE ROUTINE -
Home safety modifications: remove tripping hazards (rugs, cords); install grab bars in bathroom; ensure adequate lighting; consider hospital bed for advanced disease; secure medications and hazardous items - ROUTINE ROUTINE -
Maintain structured daily routine with consistent sleep-wake schedule, regular mealtimes, and familiar environment to reduce confusion and agitation - ROUTINE ROUTINE -

═══════════════════════════════════════════════════════════════ SECTION B: REFERENCE (Expand as Needed) ═══════════════════════════════════════════════════════════════

5. DIFFERENTIAL DIAGNOSIS

Alternative Diagnosis Key Distinguishing Features Tests to Differentiate
Rapidly progressive Alzheimer disease (rpAD) Most common misdiagnosis of CJD; positive AD biomarkers (low Abeta42, elevated p-tau); no myoclonus typically early on; MRI shows atrophy without DWI cortical ribboning; slower course (years not months) CSF AD biomarkers (Abeta42/Abeta40 ratio low, p-tau elevated, p-tau/t-tau ratio higher than CJD); RT-QuIC negative; MRI without cortical ribboning
Autoimmune encephalitis (anti-NMDAR, anti-LGI1) Subacute onset; psychiatric symptoms prominent (NMDAR); faciobrachial dystonic seizures (LGI1); steroid-responsive; CSF pleocytosis common Autoimmune encephalitis antibody panel (CSF and serum); MRI mesial temporal signal (LGI1); response to immunotherapy; RT-QuIC negative
Hashimoto encephalopathy (SREAT) Elevated anti-TPO; steroid-responsive; cognitive fluctuation; seizures; may mimic CJD closely; diagnosis of exclusion Anti-TPO and anti-thyroglobulin antibodies; empiric steroid trial (dramatic improvement supports diagnosis); RT-QuIC negative
CNS lymphoma (primary or intravascular) B symptoms; enhancing mass (primary CNS lymphoma); multifocal white matter changes + elevated LDH (intravascular lymphoma); no myoclonus MRI with contrast (enhancement); CSF cytology + flow cytometry; LDH; peripheral blood smear; brain biopsy
Neurosyphilis RPR/VDRL positive; Argyll Robertson pupils; psychiatric symptoms; may have CSF pleocytosis RPR/VDRL (serum); CSF VDRL; FTA-ABS; LP with CSF analysis
Viral encephalitis (HSV, others) Acute onset; fever; CSF pleocytosis; MRI temporal lobe signal (HSV); seizures HSV PCR (CSF); viral panels; MRI temporal enhancement; CSF pleocytosis present
Leptomeningeal carcinomatosis Known malignancy history; cranial neuropathies; communicating hydrocephalus CSF cytology (repeat x3); MRI with contrast (leptomeningeal enhancement); known primary malignancy
CNS vasculitis Headache; multifocal deficits; strokes; CSF pleocytosis; ESR/CRP elevated MRA (beading); DSA; ESR/CRP; brain/meningeal biopsy; ANCA
Dementia with Lewy bodies (DLB) Visual hallucinations; parkinsonism; REM sleep behavior disorder; fluctuating cognition; slower progression DaT scan (reduced dopamine transporter); clinical criteria; response to cholinesterase inhibitors; no DWI cortical ribboning
Toxic/metabolic encephalopathy Medication toxicity; substance abuse; hepatic/uremic/electrolyte encephalopathy; reversible Drug levels; LFTs; ammonia; BUN/Cr; UDS; correction of metabolic derangement improves cognition
Non-convulsive status epilepticus (NCSE) Altered consciousness; may have subtle motor signs; EEG shows electrographic seizures not periodic discharges Continuous EEG (seizure activity vs PSWCs); response to benzodiazepine trial; MRI without CJD pattern
Normal pressure hydrocephalus (NPH) Triad: gait (predominant) + urinary incontinence + dementia; ventricular enlargement disproportionate to atrophy Large-volume LP with pre/post gait assessment; MRI (Evans index >0.3, disproportionate ventriculomegaly)
Fatal familial insomnia (FFI) Genetic prion disease (PRNP D178N-129M); progressive insomnia; autonomic dysfunction; late motor/cognitive decline; family history PRNP gene testing; polysomnography (absent sleep spindles); FDG-PET (thalamic hypometabolism)
Gerstmann-Straussler-Scheinker syndrome (GSS) Genetic prion disease; cerebellar ataxia predominant; slower course (2-10 years); family history PRNP gene testing (P102L most common); cerebellar atrophy on MRI; slower than sCJD

6. MONITORING PARAMETERS

Parameter Frequency Target/Threshold Action if Abnormal ED HOSP OPD ICU
Cognitive assessment (MMSE, MoCA, or bedside exam) Baseline; weekly inpatient; each outpatient visit Document trajectory (decline expected in CJD) Document rate of decline; adjust prognosis; guide goals of care STAT STAT ROUTINE STAT
Neurologic exam (myoclonus, focal signs, gait, cranial nerves, akinetic mutism) Daily inpatient; each outpatient visit Document progression pattern Adjust symptomatic medications (clonazepam for myoclonus); update prognosis STAT STAT ROUTINE STAT
Swallowing assessment (SLP bedside or videofluoroscopy) Weekly inpatient; each outpatient visit; urgent if coughing with meals Safe oral intake Modified diet texture; NG tube or PEG tube discussion if progressive; aspiration precautions - ROUTINE ROUTINE ROUTINE
Functional status (ADLs, Barthel Index) Weekly inpatient; each outpatient visit Document trajectory Increase caregiver support; modify care plan; facility placement if home care insufficient - ROUTINE ROUTINE -
Seizure monitoring Continuous clinical observation; EEG if suspected subclinical seizures No clinical or electrographic seizures Adjust ASM dosing; add second agent; cEEG if unclear STAT STAT ROUTINE STAT
Myoclonus severity assessment Daily inpatient; each outpatient visit Tolerable myoclonus not interfering with comfort or function Titrate clonazepam; add valproic acid; adjust doses - ROUTINE ROUTINE ROUTINE
Respiratory status Each shift inpatient; each visit outpatient Adequate oxygenation; no aspiration pneumonia Chest X-ray if fever/tachypnea; antibiotics for aspiration PNA (if consistent with goals of care); supplemental O2 STAT ROUTINE ROUTINE STAT
Weight and nutritional status Weekly inpatient; each outpatient visit Stable or expected decline trajectory Nutritional supplementation; feeding tube discussion; palliative nutrition approach - ROUTINE ROUTINE -
Skin integrity (pressure injury assessment) Daily (inpatient); each visit (outpatient) No pressure injuries Pressure-relieving surfaces; repositioning schedule; wound care if needed - ROUTINE ROUTINE -
Caregiver burden assessment Each outpatient visit; at discharge Caregiver coping; not in crisis Respite care; support groups; social work referral; facility placement if caregiver unable to continue - ROUTINE ROUTINE -
Safety assessment (fall risk, wandering, capacity) Daily inpatient; each outpatient visit Safe environment Increase supervision; bed alarm; sitters; facility placement STAT ROUTINE ROUTINE STAT
Valproic acid level (if prescribed) Weekly until stable; then q1-3 months 50-100 mcg/mL Dose adjustment; LFTs; ammonia - ROUTINE ROUTINE -

7. DISPOSITION CRITERIA

Disposition Criteria
Discharge home Stable symptoms with adequate 24/7 caregiver; myoclonus and seizures controlled; safe swallowing or appropriate feeding plan; hospice/palliative care arranged; outpatient neurology follow-up within 1-2 weeks; advance directives completed
Admit to hospital New diagnosis requiring urgent workup (LP, MRI, EEG); uncontrolled seizures or myoclonus; acute decline requiring medication adjustment; aspiration pneumonia (if consistent with goals of care); caregiver inability to manage at home; goals of care discussion needed
Admit to ICU Status epilepticus; respiratory failure requiring mechanical ventilation (if consistent with goals of care); severe autonomic instability; refractory agitation requiring continuous sedation
Transfer to tertiary center Prion disease expertise for diagnostic confirmation; clinical trial enrollment; complex diagnostic workup (brain biopsy consideration); genetic counseling for familial forms
Memory care / Long-term care facility Progressive functional decline exceeding home caregiver capacity; safety concerns (falls, wandering, aspiration) not manageable at home
Hospice (home or inpatient) Confirmed CJD diagnosis with prognosis <6 months (applies to most sCJD at diagnosis); patient/family goals aligned with comfort-focused care; symptom management as primary goal

8. EVIDENCE & REFERENCES

Recommendation Evidence Level Source
RT-QuIC for CJD diagnosis (sensitivity 92%, specificity 99-100%) -- gold standard CSF prion biomarker Class I, Level A McGuire et al. (Ann Neurol 2012); Atarashi et al. (Nat Med 2011)
MRI DWI cortical ribboning for CJD diagnosis (sensitivity >90%) Class I, Level A Vitali et al. (Neurology 2011); Young et al. (AJNR 2005)
CSF 14-3-3 protein for CJD (sensitivity ~90%, specificity 70-85%) Class IIa, Level B Muayqil et al. (BMC Neurol 2012); WHO diagnostic criteria
CSF total tau >1150 pg/mL for CJD diagnosis (sensitivity 90%, specificity 86%) Class IIa, Level B Hamlin et al. (Neurology 2012)
CDC diagnostic criteria for probable sporadic CJD (updated 2018) Class I, Level A CDC Prion Disease - Diagnostic Criteria
WHO surveillance criteria for CJD classification (definite, probable, possible) Class I, Level A WHO Manual for Surveillance of Human Transmissible Spongiform Encephalopathies (2003)
EEG periodic sharp wave complexes in CJD -- sensitivity ~65% (highest in MM1 subtype) Class IIa, Level B Steinhoff et al. (Clin Neurophysiol 2004)
PRNP codon 129 polymorphism influences CJD phenotype and susceptibility Class I, Level B Parchi et al. (Ann Neurol 1999)
Molecular classification of sCJD subtypes (MM1, VV2, MV2K, etc.) Class I, Level B Parchi et al. (Ann Neurol 1999); Parchi et al. (Brain 2012)
Clonazepam for CJD-associated myoclonus Class IIb, Level C Expert consensus; Brown et al. (Neurology 1986)
No disease-modifying treatment exists for CJD; multiple clinical trials have failed Class I, Level A Stewart et al. (Neurology 2008) -- quinacrine trial; Geschwind et al. (Ann Neurol 2013) -- quinacrine RCT
Rapidly progressive AD is the most common misdiagnosis of CJD Class IIa, Level B Paterson et al. (Brain 2012); NPDPSC autopsy series data
Prion decontamination requires NaOH + extended autoclaving; standard sterilization insufficient Class I, Level A WHO Infection Control Guidelines for TSE (2000)
Pulvinar sign (bilateral pulvinar high signal on MRI) characteristic of variant CJD Class I, Level B Zeidler et al. (Lancet 2000)
National Prion Disease Pathology Surveillance Center (NPDPSC) for diagnostic referral and autopsy Class I, Level C NPDPSC (Case Western Reserve University)
Serum neurofilament light chain (NfL) markedly elevated in CJD; emerging blood biomarker Class IIa, Level B Thompson et al. (Ann Clin Transl Neurol 2018)
CSF p-tau/t-tau ratio distinguishes CJD from Alzheimer disease Class IIa, Level B Skillback et al. (J Alzheimers Dis 2014)
Palliative care approach recommended for all confirmed CJD patients at diagnosis Class I, Level C Expert consensus; Mead et al. (Prion 2013)
Nasal brushing RT-QuIC as emerging non-invasive prion diagnostic (sensitivity ~97%) Class IIa, Level B Orru et al. (NEJM 2014); Bongianni et al. (NEJM 2017)

CHANGE LOG

v1.1 (January 30, 2026) - Fixed structured dosing format across all treatment sections (3A, 3B, 3C) to use standard [dose] :: [route] :: [frequency] :: [full_instructions] pattern per C1/C2/C3/M1 - Reordered lab table columns (1A, 1B, 1C) to place venue columns (ED/HOSP/OPD/ICU) last per style guide per R8 - Reordered imaging table columns (2A, 2B, 2C) to place venue columns last per style guide - Added ICU column to LP table with appropriate priorities per S1/R2 - Reordered LP table columns to place venue columns last per R9 - Added ICU column to Section 4B (Patient and Family Instructions) per S2/R3 - Added ICU column to Section 4C with appropriate priorities per S3/R3 - Renamed Section 4C from "Infection Control & Safety" to "Infection Control, Safety & Lifestyle" per R4 - Added 3 lifestyle/prevention recommendations to Section 4C (caregiver self-care, home safety modifications, structured daily routine) per R5 - Fixed Haloperidol redundant dosing field (removed duplicate dose range) per M3/R6 - Standardized Section 3D palliative care entries with structured dosing format per M4/R7 - Fixed Morphine oral monitoring field (removed cross-reference "Same as IV morphine")

v1.0 (January 30, 2026) - Initial template creation - Comprehensive CJD plan covering all 4 subtypes (sporadic, familial, iatrogenic, variant) - Full 8-section format with all subsections - Structured dosing format with :: delimiters - Standardized treatment tables with Route and Indication columns - Prion-specific infection control section (Section 4C) - Palliative care emphasis throughout - NPDPSC referral and autopsy guidance - WHO/CDC diagnostic criteria in appendices - PubMed citation links for all references


APPENDIX A: CDC DIAGNOSTIC CRITERIA FOR SPORADIC CJD (2018 Update)

Definite sCJD

  • Diagnosed by standard neuropathological techniques; and/or
  • Immunocytochemically confirmed PrP-positive; and/or
  • Western blot confirmed protease-resistant PrP; and/or
  • Presence of scrapie-associated fibrils

Probable sCJD

Requires: Progressive neuropsychiatric disorder AND at least ONE of: 1. Positive RT-QuIC (CSF or other tissue) 2. Positive 14-3-3 CSF AND clinical duration <2 years 3. Typical MRI signal (caudate/putamen OR >=2 cortical regions on DWI/FLAIR) 4. Typical EEG (periodic sharp wave complexes)

PLUS at least TWO of the following clinical features: - Myoclonus - Visual or cerebellar disturbance - Pyramidal or extrapyramidal dysfunction - Akinetic mutism

AND routine investigations should not suggest an alternative diagnosis.

Possible sCJD

  • Progressive dementia
  • Duration <2 years
  • At least TWO of the four clinical features above
  • No positive result on any of the four diagnostic tests
  • Routine investigations do not suggest alternative diagnosis

APPENDIX B: VARIANT CJD (vCJD) DIAGNOSTIC CRITERIA

Definite vCJD

  • Confirmed by neuropathological examination (florid plaques, PrP immunostaining pattern)

Probable vCJD

I. Clinical criteria (ALL 5 required): 1. Progressive neuropsychiatric disorder 2. Duration >6 months 3. Routine investigations do not suggest an alternative diagnosis 4. No history of potential iatrogenic exposure 5. No evidence of familial form of TSE

II. Clinical features (at least 4 of 5): - Early psychiatric symptoms (depression, anxiety, apathy, withdrawal, delusions) - Persistent painful sensory symptoms (pain, dysesthesia) - Ataxia - Myoclonus or chorea or dystonia - Dementia

III. Diagnostic tests: - EEG does NOT show typical sCJD pattern (PSWCs usually absent in vCJD) - Pulvinar sign on MRI (bilateral pulvinar high signal, sensitivity ~90%) - Tonsil biopsy positive for PrP (if performed)

APPENDIX C: PRNP CODON 129 GENOTYPE AND sCJD SUBTYPES

Subtype Codon 129 PrP Type Clinical Phenotype Typical Duration
MM1 (most common, ~70%) MM Type 1 Classic: rapid cognitive decline, myoclonus, PSWCs on EEG, cortical ribboning on MRI 3-4 months
VV2 VV Type 2 Ataxic variant: prominent cerebellar ataxia early; dementia later; longer course 6-7 months
MV2K MV Type 2 Kuru-type: ataxia, dementia, prolonged course; may lack typical EEG findings 17-18 months
MM2C MM Type 2 Cortical: progressive dementia without typical EEG or MRI in early stages 15-16 months
MM2T MM Type 2 Thalamic (sporadic fatal insomnia): insomnia, autonomic dysfunction, resembles FFI 15-30 months
VV1 (rare) VV Type 1 Early onset; slowly progressive dementia; may lack myoclonus 15-21 months

Note: Codon 129 MM homozygosity is a risk factor for sCJD and is present in ~70% of sCJD cases (vs ~37% of the general population). The molecular classification combines codon 129 genotype with PrP type (determined at autopsy) to define clinicopathological subtypes with distinct clinical presentations and prognosis.

APPENDIX D: PRION DECONTAMINATION PROTOCOLS

Tissue Category Infectivity Level Decontamination Required
Brain, spinal cord, posterior eye HIGH WHO-recommended: 1N NaOH for 1 hour at room temperature followed by autoclaving at 134 degrees C for 1 hour in gravity displacement autoclave; OR destroy instruments
CSF, anterior eye, olfactory mucosa LOW-MODERATE Standard precautions for handling; standard sterilization generally acceptable for instruments not contacting tissue directly
Blood, urine, feces, saliva, skin LOW/NEGLIGIBLE Standard precautions only; no special decontamination
Environmental surfaces NEGLIGIBLE Standard cleaning; 1N NaOH or 20,000 ppm sodium hypochlorite for 1 hour if contaminated with high-infectivity tissue

Key Principles: - Standard autoclaving (121 degrees C for 15-30 min) does NOT reliably inactivate prions - Formaldehyde, ethanol, ethylene oxide, UV light, and ionizing radiation do NOT inactivate prions - Single-use disposable instruments are PREFERRED for any invasive procedure on CJD patients - Contact hospital infection control before ANY invasive procedure on confirmed or suspected CJD patients